DhelixGene WSSV(White Spot Syndrome Virus) qPCR detection kit for shrimp disease diagnosis

White Spot Syndrome Virus (WSSV) Detection Kit

White Spot Syndrome Virus (WSSV) Detection Kit by DHelixGene is a highly sensitive real-time PCR (qPCR)-based kit for rapid pathogen detection. Ideal for shrimp farming and aquaculture diagnostics. Get accurate results in 45 minutes.

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The Gold Standard in Aquaculture Biosecurity：

PCR technology has been widely applied in the aquaculture industry for disease surveillance, early diagnosis, and health management. With the increasing demand for high-throughput screening, quantitative analysis, and rapid decision-making, real-time PCR（qPCR） has become an essential tool for modern aquaculture biosecurity.

The Dhelix Aquatic Pathogen Detection System is a real-time PCR-based detection solution designed for accurate and reliable identification of major aquatic pathogens. By integrating advanced assay design and optimized reaction chemistry, this system provides a highly sensitive, specific, and flexible detection platform, supporting disease control and improving production efficiency in aquaculture operations.Compared to traditional methods, our TaqMan probe-based qPCR ensures zero cross-reactivity and maximum accuracy, making it the gold standard for aquaculture biosafety.

PCR technology has been widely applied in the aquaculture industry for disease surveillance, early diagnosis, and health management. With the increasing demand for high-throughput screening, quantitative analysis, and rapid decision-making, real-time PCR（qPCR） has become an essential tool for modern aquaculture biosecurity.

The Dhelix Aquatic Pathogen Detection System is a real-time PCR-based detection solution designed for accurate and reliable identification of major aquatic pathogens. By integrating advanced assay design and optimized reaction chemistry, this system provides a highly sensitive, specific, and flexible detection platform, supporting disease control and improving production efficiency in aquaculture operations.Compared to traditional methods, our TaqMan probe-based qPCR ensures zero cross-reactivity and maximum accuracy, making it the gold standard for aquaculture biosafety.

Advantages of DHelix System ：

Rapid Results

Accelerated workflow provides accurate testing data in under 2 hours.

Exceptional Specificity

TaqMan® probe-based strategy ensures zero cross-reactivity.

High Sensitivity

Detects low copy numbers of target pathogens for early warning.

User-friendly

Simplified operation requiring minimal hands-on time and training.

Standardized Workflow

Ready-to-use reagents ensure consistency across batches and labs.

GOLD Standard

Advanced TaqMan® technology for maximum diagnostic confidence.

Technical Features & System Highlights ：

TaqMan® Probe-based Detection Strategy:
The system utilizes a probe-based real-time PCR approach, enabling higher specificity and improved tolerance to complex sample matrices compared to dye-based systems. It allows accurate detection even in samples with low pathogen load or high background interference.

Multiplex Detection Design:
This system supports simultaneous detection of multiple targets within a single reaction (e.g., pathogen gene + internal control). The built-in internal control effectively monitors nucleic acid extraction and amplification efficiency, minimizing the risk of false-negative results.

High Sensitivity and Broad Dynamic Range:
The assay is capable of detecting low copy numbers of target nucleic acids with excellent linearity across a wide dynamic range, ensuring reliable performance in both early infection and high-load conditions.

Flexible Quantification Methods:
The system supports both absolute quantification (copy number per reaction or per unit volume) and relative quantification strategies, allowing users to evaluate infection levels under different application scenarios.

Robust Performance and Reproducibility:
Optimized primers, probes, and reaction conditions ensure high amplification efficiency and consistent results across different batches, instruments, and operators.

User-friendly Workflow:
The ready-to-use reagent format simplifies operation procedures, reduces hands-on time, and minimizes contamination risk, making it suitable for routine laboratory testing as well as field laboratories.

TaqMan® Probe-based Detection Strategy:
The system utilizes a probe-based real-time PCR approach, enabling higher specificity and improved tolerance to complex sample matrices compared to dye-based systems. It allows accurate detection even in samples with low pathogen load or high background interference.

Multiplex Detection Design:
This system supports simultaneous detection of multiple targets within a single reaction (e.g., pathogen gene + internal control). The built-in internal control effectively monitors nucleic acid extraction and amplification efficiency, minimizing the risk of false-negative results.

High Sensitivity and Broad Dynamic Range:
The assay is capable of detecting low copy numbers of target nucleic acids with excellent linearity across a wide dynamic range, ensuring reliable performance in both early infection and high-load conditions.

Flexible Quantification Methods:
The system supports both absolute quantification (copy number per reaction or per unit volume) and relative quantification strategies, allowing users to evaluate infection levels under different application scenarios.

Robust Performance and Reproducibility:
Optimized primers, probes, and reaction conditions ensure high amplification efficiency and consistent results across different batches, instruments, and operators.

User-friendly Workflow:
The ready-to-use reagent format simplifies operation procedures, reduces hands-on time, and minimizes contamination risk, making it suitable for routine laboratory testing as well as field laboratories.

Pathogen Overview ：

White Spot Syndrome Virus (WSSV) is arguably the most lethal pathogen in the global shrimp farming industry, capable of causing up to 100% mortality within 3 to 7 days of infection. Characterized by visible white calcium deposits on the carapace, WSSV spreads rapidly through waterborne transmission and infected prey. Due to its catastrophic impact on Litopenaeus vannamei and other penaeid species, high-sensitivity qPCR screening is the gold standard for early detection to prevent farm-wide outbreaks.

White Spot Syndrome Virus (WSSV) is arguably the most lethal pathogen in the global shrimp farming industry, capable of causing up to 100% mortality within 3 to 7 days of infection. Characterized by visible white calcium deposits on the carapace, WSSV spreads rapidly through waterborne transmission and infected prey. Due to its catastrophic impact on Litopenaeus vannamei and other penaeid species, high-sensitivity qPCR screening is the gold standard for early detection to prevent farm-wide outbreaks.

Technical Specifications：

  • Catalog No.：031012RME
  • Format： 48 T
  • Description： This kit is used for the detection of White Spot Syndrome Virus (WSSV).
  • Sample Types： shrimp、 larvae、bait、Processed aquatic products etc.
  • Protocol: 1、Sample Preparation；2、Reagents Preparation；3、Amplification reaction；

1. This reagent kit should be stored at -20°C. To prevent the reagents from deterioration, only take out the necessary amount of reagents from the freezer before use (in order to maintain the reagents performance, avoid unnecessary freezing and thawing).

2. Thaw the reagents at room temperature. Before use, spin down the tubes to drop down the reagents staying on the tube wall or on the cap, and mix well the reagents and spin down again. Notice that fierce mixing should be avoided as it can inactivate the Bst DNA polymerase.

3.Positive Control( PG-P1-I ) contains a high number of copies. In order to prevent Positive Control from contaminating other samples or reagents, always spin down before opening the tube and open the cap of the tube as short as possible. Also add into the reaction tubes under the following order from blank control, sample solution (extracted RNA), and leave the adding of positive control ( PG-P1-I) to the last and make sure that all other tube caps are closed when adding it. Moreover, to avoid contamination, do not use Positive Control ( PG-P1-I) in any other way not written in this instruction (such as diluting the positive control or adding it to samples)。

4. Keep positive control and suspect positive samples away from the reagents when handling

5. If there is any reagent left, do not use it with other kits even if they are in the same lot

6. The caps of the used reaction tubes should not be opened. Pay special attention not to accidentally open the cap when taking the tubes out of the instrument. Contamination of amplified products on other samples may not only cause false judgment of the test result but also pollute the testing area. In this case, a correct test result may not be obtained unless pollution is completely removed.

7. Keep the cap of the used tube completely closed and after double bagging it with the sealable vinyl bag. To prevent the amplified products from dispersing, do not conduct autoclave sterilization treatment for disposal.

8.This kit should be stored at -20°C. Storing the kit at a temperature lower than -20°C or repeated freezing and thawing might cause cracks on the tubes.

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