African Swine Fever Virus(ASFV) Detection Kit
African Swine Fever Virus (ASFV) Detection Kit by DHelixGene is a highly sensitive real-time PCR (qPCR)-based kit for rapid pathogen detection. Ideal for livestock biosecurity, farm environment internal controls, and swine disease diagnostics. Get accurate, actionable results in 45 minutes.
Real-Time qPCR Technology for Livestock Biosecurity & Disease Surveillance:
PCR technology has been widely applied in the livestock industry for disease surveillance, early diagnosis, and herd health management. With the increasing demand for high-throughput screening, quantitative analysis, and rapid herd-level decision-making, real-time PCR (qPCR) has become an essential tool for modern livestock biosecurity and farm-level internal controls.The DHelix Livestock Pathogen Detection System is a real-time PCR-based detection solution designed for accurate and reliable identification of major swine and livestock pathogens. By integrating advanced assay design and optimized reaction chemistry, this system provides a highly sensitive, specific, and flexible detection platform, supporting infectious disease control and improving production stability in commercial farming operations.Compared to traditional methods, our TaqMan probe-based qPCR ensures zero cross-reactivity and maximum accuracy, making it the definitive gold standard for global livestock biosafety and environmental self-screening.
Real-Time qPCR Technology for Livestock Biosecurity & Disease Surveillance:
PCR technology has been widely applied in the livestock industry for disease surveillance, early diagnosis, and herd health management. With the increasing demand for high-throughput screening, quantitative analysis, and rapid herd-level decision-making, real-time PCR (qPCR) has become an essential tool for modern livestock biosecurity and farm-level internal controls.The DHelix Livestock Pathogen Detection System is a real-time PCR-based detection solution designed for accurate and reliable identification of major swine and livestock pathogens. By integrating advanced assay design and optimized reaction chemistry, this system provides a highly sensitive, specific, and flexible detection platform, supporting infectious disease control and improving production stability in commercial farming operations.Compared to traditional methods, our TaqMan probe-based qPCR ensures zero cross-reactivity and maximum accuracy, making it the definitive gold standard for global livestock biosafety and environmental self-screening.
Advantages of DHelix System ：
Rapid Results
Accelerated workflow provides accurate testing data in under 2 hours.
Exceptional Specificity
TaqMan® probe-based strategy ensures zero cross-reactivity.
High Sensitivity
Detects low copy numbers of target pathogens for early warning.
User-friendly
Simplified operation requiring minimal hands-on time and training.
Standardized Workflow
Ready-to-use reagents ensure consistency across batches and labs.
GOLD Standard
Advanced TaqMan® technology for maximum diagnostic confidence.
Technical Features & System Highlights ：
TaqMan® Probe-based Detection Strategy:
The system utilizes a probe-based real-time PCR approach, enabling higher specificity and improved tolerance to complex sample matrices compared to dye-based systems. It allows accurate detection even in samples with low pathogen load or high background interference.
Multiplex Detection Design:
This system supports simultaneous detection of multiple targets within a single reaction (e.g., pathogen gene + internal control). The built-in internal control effectively monitors nucleic acid extraction and amplification efficiency, minimizing the risk of false-negative results.
High Sensitivity and Broad Dynamic Range:
The assay is capable of detecting low copy numbers of target nucleic acids with excellent linearity across a wide dynamic range, ensuring reliable performance in both early infection and high-load conditions.
Flexible Quantification Methods:
The system supports both absolute quantification (copy number per reaction or per unit volume) and relative quantification strategies, allowing users to evaluate infection levels under different application scenarios.
Robust Performance and Reproducibility:
Optimized primers, probes, and reaction conditions ensure high amplification efficiency and consistent results across different batches, instruments, and operators.
User-friendly Workflow:
The ready-to-use reagent format simplifies operation procedures, reduces hands-on time, and minimizes contamination risk, making it suitable for routine laboratory testing as well as field laboratories.
Technical Features & System Highlights （(Click to Expand)）：
TaqMan® Probe-based Detection Strategy:
The system utilizes a probe-based real-time PCR approach, enabling higher specificity and improved tolerance to complex sample matrices compared to dye-based systems. It allows accurate detection even in samples with low pathogen load or high background interference.
Multiplex Detection Design:
This system supports simultaneous detection of multiple targets within a single reaction (e.g., pathogen gene + internal control). The built-in internal control effectively monitors nucleic acid extraction and amplification efficiency, minimizing the risk of false-negative results.
High Sensitivity and Broad Dynamic Range:
The assay is capable of detecting low copy numbers of target nucleic acids with excellent linearity across a wide dynamic range, ensuring reliable performance in both early infection and high-load conditions.
Flexible Quantification Methods:
The system supports both absolute quantification (copy number per reaction or per unit volume) and relative quantification strategies, allowing users to evaluate infection levels under different application scenarios.
Robust Performance and Reproducibility:
Optimized primers, probes, and reaction conditions ensure high amplification efficiency and consistent results across different batches, instruments, and operators.
User-friendly Workflow:
The ready-to-use reagent format simplifies operation procedures, reduces hands-on time, and minimizes contamination risk, making it suitable for routine laboratory testing as well as field laboratories.
Pathogen Overview ：
African Swine Fever Virus (ASFV) is a highly contagious, severe hemorrhagic viral disease affecting domestic and wild pigs, capable of inflicting up to 100% mortality in infected herds. Because there is no universally available, fully effective commercial treatment, robust farm-level biosecurity relies entirely on “early detection and rapid stamping-out.” The virus is exceptionally resilient, surviving for months in the environment, transport vehicles, and feed ingredients. Traditional clinical observation only identifies the threat after massive viral shedding has occurred. DHelixGene’s qPCR assay targets the highly conserved p72 gene region within the ASFV viral DNA payload, maximizing analytical sensitivity down to extremely low copy numbers. It offers a precise diagnostic solution that delivers actionable data in 45 minutes, empowering livestock facility directors to execute routine zero-tolerance vehicle screening, environmental swabbing, and early farm-to-fork internal control vetting effectively.
Pathogen Overview ：(Click to Expand)：
African Swine Fever Virus (ASFV) is a highly contagious, severe hemorrhagic viral disease affecting domestic and wild pigs, capable of inflicting up to 100% mortality in infected herds. Because there is no universally available, fully effective commercial treatment, robust farm-level biosecurity relies entirely on “early detection and rapid stamping-out.” The virus is exceptionally resilient, surviving for months in the environment, transport vehicles, and feed ingredients. Traditional clinical observation only identifies the threat after massive viral shedding has occurred. DHelixGene’s qPCR assay targets the highly conserved p72 gene region within the ASFV viral DNA payload, maximizing analytical sensitivity down to extremely low copy numbers. It offers a precise diagnostic solution that delivers actionable data in 45 minutes, empowering livestock facility directors to execute routine zero-tolerance vehicle screening, environmental swabbing, and early farm-to-fork internal control vetting effectively.
Technical Specifications：
- Catalog No.：036022ME
- Format： 48 T
- Description： This kit is used for the detection of African Swine Fever Virus(ASFV).
- Sample Types：Highly Validated Testing Matrices:Biological & Clinical Samples: Whole blood, plasma, hemoglobin powder, and infected tissue biopsies (lymph nodes, spleen, tonsils).Commercial Swine Feed Internal Controls: Complete mash/pellet feeds, raw feed ingredients, and commercial nutritional additives.Supply Chain Pork Product Screening: Processed retail pork products, including hams, cured sausages, and raw/cooked meat fillings. etc.
- Protocol: 1、Sample Preparation；2、Reagents Preparation；3、Amplification reaction；
Handling Precautions & Storage(Click to Expand)：
1. This reagent kit should be stored at -20°C. To prevent the reagents from deterioration, only take out the necessary amount of reagents from the freezer before use (in order to maintain the reagents performance, avoid unnecessary freezing and thawing).
2. Thaw the reagents at room temperature. Before use, spin down the tubes to drop down the reagents staying on the tube wall or on the cap, and mix well the reagents and spin down again. Notice that fierce mixing should be avoided as it can inactivate the Taq DNA Polymerase.
3.Positive Control( ASFV Positive Control) contains a high number of copies. In order to prevent Positive Control from contaminating other samples or reagents, always spin down before opening the tube and open the cap of the tube as short as possible. Also add into the reaction tubes under the following order from blank control, sample solution (extracted DNA), and leave the adding of positive control (ASFV Positive Control) to the last and make sure that all other tube caps are closed when adding it. Moreover, to avoid contamination, do not use Positive Control (ASFV Positive Control) in any other way not written in this instruction (such as diluting the positive control or adding it to samples)。
4. Keep positive control and suspect positive samples away from the reagents when handling
5. If there is any reagent left, do not use it with other kits even if they are in the same lot
6. The caps of the used reaction tubes should not be opened. Pay special attention not to accidentally open the cap when taking the tubes out of the instrument. Contamination of amplified products on other samples may not only cause false judgment of the test result but also pollute the testing area. In this case, a correct test result may not be obtained unless pollution is completely removed.
7. Keep the cap of the used tube completely closed and after double bagging it with the sealable vinyl bag. To prevent the amplified products from dispersing, do not conduct autoclave sterilization treatment for disposal.
8.This kit should be stored at -20°C. Storing the kit at a temperature lower than -20°C or repeated freezing and thawing might cause cracks on the tubes.
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