Bovine-Derived Component Nucleic Acid Detection Kit
Bovine-Derived Component Nucleic Acid Detection Kit by DHelixGene is a highly sensitive real-time PCR (qPCR)-based kit for rapid animal origin tracing. Ideal for Halal food compliance, feed raw material internal controls, and meat authenticity verification. Get accurate, actionable results in 45 minutes.
Real-Time qPCR Technology for Meat Authenticity & Halal Integrity Verification：
PCR technology has been widely applied in the food and feed processing industries for ingredient traceability, material authentication, and supply chain integrity management. With the increasing global demand for high-throughput screening, quantitative analysis, and rapid batch-level decision-making, real-time PCR (qPCR) has become an essential tool for modern livestock feed internal controls and regulatory Halal compliance.The DHelix Meat-Derived Component Identification System is a real-time PCR-based molecular solution designed for accurate and reliable tracing of specific animal constituents in commercial food and raw materials. By integrating advanced primer-probe design and optimized reaction chemistry, this system provides a highly sensitive, specific, and flexible verification platform, supporting raw material validation and safeguarding brand reputation in commercial processing operations.Compared to traditional methods, our TaqMan probe-based qPCR ensures zero cross-reactivity and maximum analytical sensitivity, making it the definitive gold standard for global food authenticity, feed adulteration self-screening, and religious dietary compliance.
Real-Time qPCR Technology for Meat Authenticity & Halal Integrity Verification：
PCR technology has been widely applied in the food and feed processing industries for ingredient traceability, material authentication, and supply chain integrity management. With the increasing global demand for high-throughput screening, quantitative analysis, and rapid batch-level decision-making, real-time PCR (qPCR) has become an essential tool for modern livestock feed internal controls and regulatory Halal compliance.The DHelix Meat-Derived Component Identification System is a real-time PCR-based molecular solution designed for accurate and reliable tracing of specific animal constituents in commercial food and raw materials. By integrating advanced primer-probe design and optimized reaction chemistry, this system provides a highly sensitive, specific, and flexible verification platform, supporting raw material validation and safeguarding brand reputation in commercial processing operations.Compared to traditional methods, our TaqMan probe-based qPCR ensures zero cross-reactivity and maximum analytical sensitivity, making it the definitive gold standard for global food authenticity, feed adulteration self-screening, and religious dietary compliance.
Advantages of DHelix System ：
Rapid Results
Accelerated workflow provides accurate testing data in under 2 hours.
Exceptional Specificity
TaqMan® probe-based strategy ensures zero cross-reactivity.
High Sensitivity
Detects low copy numbers of target pathogens for early warning.
User-friendly
Simplified operation requiring minimal hands-on time and training.
Standardized Workflow
Ready-to-use reagents ensure consistency across batches and labs.
GOLD Standard
Advanced TaqMan® technology for maximum diagnostic confidence.
Technical Features & System Highlights ：
TaqMan® Probe-based Detection StrategyThe system utilizes a probe-based real-time PCR approach, enabling higher specificity and improved tolerance to complex sample matrices compared to dye-based systems. It allows accurate detection even in samples with low target copy numbers or high background interference.Multiplex Detection DesignThis system supports simultaneous detection of multiple targets within a single reaction (e.g., target gene + internal control). The built-in internal control effectively monitors nucleic acid extraction and amplification efficiency, minimizing the risk of false-negative results.High Sensitivity and Broad Dynamic RangeThe assay is capable of detecting low copy numbers of target nucleic acids with excellent linearity across a wide dynamic range, ensuring reliable performance in both trace contamination screening and high-load verification conditions.Flexible Quantification MethodsThe system supports both absolute quantification (copy number per reaction or per unit volume) and relative quantification strategies, allowing users to evaluate presence and concentration levels under different application scenarios.Robust Performance and ReproducibilityOptimized primers, probes, and reaction conditions ensure high amplification efficiency and consistent results across different batches, instruments, and operators.User-friendly WorkflowThe ready-to-use reagent format simplifies operation procedures, reduces hands-on time, and minimizes contamination risk, making it suitable for routine laboratory testing as well as field laboratories.
Technical Features & System Highlights （(Click to Expand)）：
TaqMan® Probe-based Detection StrategyThe system utilizes a probe-based real-time PCR approach, enabling higher specificity and improved tolerance to complex sample matrices compared to dye-based systems. It allows accurate detection even in samples with low target copy numbers or high background interference.Multiplex Detection DesignThis system supports simultaneous detection of multiple targets within a single reaction (e.g., target gene + internal control). The built-in internal control effectively monitors nucleic acid extraction and amplification efficiency, minimizing the risk of false-negative results.High Sensitivity and Broad Dynamic RangeThe assay is capable of detecting low copy numbers of target nucleic acids with excellent linearity across a wide dynamic range, ensuring reliable performance in both trace contamination screening and high-load verification conditions.Flexible Quantification MethodsThe system supports both absolute quantification (copy number per reaction or per unit volume) and relative quantification strategies, allowing users to evaluate presence and concentration levels under different application scenarios.Robust Performance and ReproducibilityOptimized primers, probes, and reaction conditions ensure high amplification efficiency and consistent results across different batches, instruments, and operators.User-friendly WorkflowThe ready-to-use reagent format simplifies operation procedures, reduces hands-on time, and minimizes contamination risk, making it suitable for routine laboratory testing as well as field laboratories.
Component Authentication：
Bovine-derived component detection is essential for verifying meat authenticity, managing economic fraud in premium beef supply chains, and ensuring compliance with livestock feed regulations (such as preventing Bovine Spongiform Encephalopathy / BSE cross-contamination). Unscrupulous mixing of meat matrices compromises product labeling and consumer trust. DHelixGene’s qPCR assay utilizes advanced primer-probe architecture targeting highly specific, multi-copy mitochondrial genes within the bovine genome, ensuring high sensitivity even in highly processed gelatin, rendered fats, or bone meals. This provides a precise diagnostic solution that delivers actionable quantitative data in 45 minutes, allowing enterprise-level meat processors and animal feed conglomerates to enforce absolute quality boundaries, clear incoming raw ingredients, and maintain supply chain biosecurity seamlessly.
Component Authentication ：(Click to Expand)：
Bovine-derived component detection is essential for verifying meat authenticity, managing economic fraud in premium beef supply chains, and ensuring compliance with livestock feed regulations (such as preventing Bovine Spongiform Encephalopathy / BSE cross-contamination). Unscrupulous mixing of meat matrices compromises product labeling and consumer trust. DHelixGene’s qPCR assay utilizes advanced primer-probe architecture targeting highly specific, multi-copy mitochondrial genes within the bovine genome, ensuring high sensitivity even in highly processed gelatin, rendered fats, or bone meals. This provides a precise diagnostic solution that delivers actionable quantitative data in 45 minutes, allowing enterprise-level meat processors and animal feed conglomerates to enforce absolute quality boundaries, clear incoming raw ingredients, and maintain supply chain biosecurity seamlessly.
Technical Specifications：
- Catalog No.：021022M
- Format： 48 T
- Description： This kit is used for the detection of Bovine-Derived Component Nucleic Acid.
- Sample Types： Validated Food & Processing Matrices:Raw & Temperature-Controlled Proteins: Raw fresh meat, premium frozen cuts, and mechanically deboned meat scraps.Traditional Cured & Cooked Delis: Cured and marinated meats (bacon, hams), braised/soy-sauced meat lines, and smoked/grilled commercial products.Shelf-Stable Products & Specialized Tissues: Canned/retorted meat items, fine meat fillings, and brain tissues or central nervous system (CNS) matrices for strict compliance screening. etc.
- Protocol: 1、Sample Preparation；2、Reagents Preparation；3、Amplification reaction；
Handling Precautions & Storage(Click to Expand)：
1. This reagent kit should be stored at -20°C. To prevent the reagents from deterioration, only take out the necessary amount of reagents from the freezer before use (in order to maintain the reagents performance, avoid unnecessary freezing and thawing).
2. Thaw the reagents at room temperature. Before use, spin down the tubes to drop down the reagents staying on the tube wall or on the cap, and mix well the reagents and spin down again. Notice that fierce mixing should be avoided as it can inactivate the Taq DNA Polymerase.
3.Positive Control( PG-P1-I ) contains a high number of copies. In order to prevent Positive Control from contaminating other samples or reagents, always spin down before opening the tube and open the cap of the tube as short as possible. Also add into the reaction tubes under the following order from blank control, sample solution (extracted DNA), and leave the adding of positive control ( PG-P1-I) to the last and make sure that all other tube caps are closed when adding it. Moreover, to avoid contamination, do not use Positive Control ( PG-P1-I) in any other way not written in this instruction (such as diluting the positive control or adding it to samples)。
4. Keep positive control and suspect positive samples away from the reagents when handling
5. If there is any reagent left, do not use it with other kits even if they are in the same lot
6. The caps of the used reaction tubes should not be opened. Pay special attention not to accidentally open the cap when taking the tubes out of the instrument. Contamination of amplified products on other samples may not only cause false judgment of the test result but also pollute the testing area. In this case, a correct test result may not be obtained unless pollution is completely removed.
7. Keep the cap of the used tube completely closed and after double bagging it with the sealable vinyl bag. To prevent the amplified products from dispersing, do not conduct autoclave sterilization treatment for disposal.
8.This kit should be stored at -20°C. Storing the kit at a temperature lower than -20°C or repeated freezing and thawing might cause cracks on the tubes.
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